Method for growth in tissue culture of normal colonic epithelial cells and method for determination of preneoplastic color cells

摘要

The invention concerns a method for the routine primary culture of human colonic epithelial cells within a monolayer. 12-0-tetradecanoylphorbol-13-acetate (TPA) enhanced DNA synthesis an average of 8 fold when assayed by [3h]dThd labeling indices (L.I.) in colonic epithelial cells from familial polyposis patients and in tubular adenomas. No such stimulation by TPA was seen in cells from high-risk patients without familial polyposis, or in cells from low-risk subjects.