| 摘要 |
PURPOSE:To produce an immobilized microbial cell durable to repeated use over a long period, by treating a microbial cell with a diamine and a dialdehyde and immobilizing the treated cell with a polymeric substance. CONSTITUTION:The cell of bacterium, yeast, fungus, etc., preferably a microbial cell having remarkable leakage of enzyme from the cell is made to contact with a diamine (e.g. ethylenediamine, hexamethylenediamine, etc.) and a dialdehyde (e.g. glyoxal, phthalaldehyde, etc.) at 1-40 deg.C and 5-8pH for about 30min-3hr. The treated cell is immobilized in a polymeric substance such as polyvinyl alcohol gel, carrageenan, etc.
|
