Use of demineralized bone matrix in the repair of segmental defects

摘要

A process of grafting material from milled bone is disclosed which comprises augmenting the bone protein of a first quantity of milled bone by dialyzing from solution guanidine-extracted bone proteins from a second quantity of milled bone and adding the extracted proteins to the first quantity of milled bone. Next, unbound proteins are removed and then the augmented milled bone is lypohilized, after which the augmented milled bone is coated with anti-coagulated plasma containing plasma proteins. Any unbound plasma protein is removed from the coating by rinsing.