IMPROVED NUCLEIC ACID HYBRIDIZATION TECHNIQUE AND KIT THEREFOR

摘要

A method for detecting a polynucleotide in a sample comprising: combining in liquid phase the sample with a first and second probe, each binding to different sequences of the target polynucleotide. The ''target/probe'' complex is immobilized subsequently with a solid carrier able to bind the first probe. The second probe carries a detectable label. A kit for use with the method and the use thereof for the diagnosis of genetic diseases, such as sickle cell anaemia, and for cancer is disclosed.