Method of producing human prourokinase.

摘要

A method of producing glycosylated single-chain prourokinase which comprises cultivating dihydrofolate reductase gene-deficient CHO-K1 cells transformed with a plasmid containing, as inserts a pro-UK cDNA sequence, prepared by using human kidney cell line-derived mRNA as template, SV40 promoter genes, and the dihydrofolate reductase gene, wherein the expression of said pro-UK cDNA is controlled by a SV40 promoter gene and the expression of said dihydrofolate reductase gene is controlled by a second SV40 promoter gene.