| 摘要 |
PURPOSE:To obtain the titled amine having high gamma-linoleic acid content and useful as a medicinal drug, by culturing a specific microbial strain of Mortierella genus in a medium containing a carbohydrate at a high concentration as a carbon source, in high microbial cell density keeping high lipid content in the cell. CONSTITUTION:A microbial strain belonging to Mortierella isabellina, vinacea, ramaniana, ramaniana anglispora or nana is inoculated in a medium containing 20-400g of a carbohydrate (e.g. molasses) per 1 liter of the medium and a nitrogen source, etc., and cultured at 3-6pH for 2-10 days adding 0.1-20g/l of acetic acid at the initial stage of cultivation. The cell having a water-content of about 60% is collected from the culture product by centrifugal dehydrator, etc. 1pt.wt. of the cell (dry basis) is added with 2-7pts.wt. of a solvent produced by mixing 1pt.wt. of an alcohol and 0.2-0.7pt.wt. of water and the cell is disintegrated and extracted. The extracted component is separated by column chromatography to obtain the objective phosphatidyl ethanolamine of formula containing >=20% gamma-linoleic acid at the COOH of the R or R'. |
