| 摘要 |
PURPOSE:In cultivating a mold belonging to the genus Mortierella, to obtain a mold having a high content of gamma-linolenic acid in high productivity, by taking out part of a medium containing growth molds having a given amount of lipid, feeding a supplement medium to the medium and repeating the culture operation. CONSTITUTION:In cultivating a mold belonging to the genus Mortierella by using a carbohydrate as a carbon source, the culture process is divided into two processes of a mold growth process and a process to accumulate lipid in a mold, part of a medium containing the mold having desired lipid is taken out from the process to accumulate lipid in the mold, lipid (gamma-linolenic acid is collected, part of the medium containing the growth mold is supplied from the mold growth process to the process to accumulate lipid in the mold and culture is carried out. |
