发明名称 |
Method for the production of proteins by means of inducible expression systems in genetically modified eukaryotic host-cells multiplicated in-vivo. |
摘要 |
<p>In the production of proteins of biological interest by means of a stress inducible gene expression unit/eukaryotic host cell system, the transformed cell lines are multiplicated by tumour growing in immunodefficient warm-bl ooded animals, after which the multiplicated cells are cultured in vitro and subjected to stress, whereby expression occurs in high yield. In vivo multiplication rates of 10<5>-10<6> the innoculated quantity/2 weeks are reported without any loss of the latent inducible expression capacity.</p> |
申请公布号 |
EP0263908(A1) |
申请公布日期 |
1988.04.20 |
申请号 |
EP19860810455 |
申请日期 |
1986.10.15 |
申请人 |
BATTELLE MEMORIAL INSTITUTE |
发明人 |
BROMLEY, PETER;DREANO, MICHEL;FISCHBAH, MICHEL;FOUILLET, XAVIER;PADIEU, PRUDENT;VOELLMY, RICHARD |
分类号 |
C12N15/09;A01K67/027;C07K14/61;C12N5/00;C12N5/10;C12N15/00;C12N15/85;C12P21/00;C12P21/02;C12R1/91;(IPC1-7):C12N15/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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