发明名称 |
Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays |
摘要 |
A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, and (c) a label chemically linked to the nucleic acid-binding ligand. The label can be a specifically bindable ligand such as a hapten or biotin, an enzyme such as a beta -galactosidase or horse radish peroxidase, a fluorescent radical, a phycobiliprotein, a luminescent radical, or a radioisotope. The probe can be used in assays of nucleic acids, taking advantage of the ability of the nucleic acid component to hybridize.
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申请公布号 |
US4737454(A) |
申请公布日期 |
1988.04.12 |
申请号 |
US19840611668 |
申请日期 |
1984.05.18 |
申请人 |
MOLECULAR DIAGNOSTICS, INC. |
发明人 |
DATTAGUPTA, NANIBHUSHAN;CROTHERS, DONALD M. |
分类号 |
C07H21/00;C12Q1/68;G01N33/533;(IPC1-7):C12Q1/68;C12Q1/00;C07H17/00;C12N15/00 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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