Regulation and enhancement of enzyme production

摘要

A method is disclosed for producing catabolite repression-resistant mutant strains of C. thermosulfurogenes and C. thermohydrosulfuricum. The method comprises challenging a wild strain of the organism with nitrosoguanidine, followed by enrichment on 2-deoxyglucose and culturing an iodine stained starch-glucose containing agar. The colonies which convert starch most efficiently are catabolite repression-resistant. Pure cultures of the mutants and methods employing the mutants to prepare enzymes are also described.