| 摘要 |
PURPOSE:To obtain an organ function improver comprising a polypeptide having substantially the same amino acid sequence as that of human superoxide dismutase (SOD) as an active ingredient. CONSTITUTION:DNA is separated from normal human tissue (e.g. liver, placenta), mRNA (mixture containing mRNA of human Cu,Zn-SOD) having poly(A)tail is separated by the use of oligo(dT)cellulose and poly(U)Sepharose and cDNA of human Cu,Zn-SOD is synthesized by using the mRNA. A self-replicative vector is integrated with a DNA fragment (preferably fragment of colicin E1 gene having positive regulation site) containing character manifestation regulating gene and a structural gene fragment of human Cu,Zn-SOD to give the aimed recombinant DNA. A bacterium transformed with the recombinant DNA is cultivated and human Cu,Zn-SOD accumulated in the culture mixture is collected. |
