| 摘要 |
Generation of competent embryoids, plantlets and plants (including seeds) from Cucumis sp. explant tissue, e.g., from cotyledonary, root, leaf of stem tissue, by induction followed by promotion of embryoid development in a series of media containing one or more of 2,4-D, BAP and NAA under defined temperature, time and light conditions. The explant tissue may be from any plant type: wild or cultivated, hybrid (somatic or sexual), or genetically variant or transformed plants. Competent embryoids may be continuously produced by serial subculture. Serial transfer to novel induction and expression media for defined periods causes sequential development of competent embryoids and plantlets. Transfer to development media permits plantlet development, which in turn progress to mature plants in hardening media and/or soil. The process is applicable to producing encapsulated embryoids form single genotypes or somatic hybrids as ''artificial seeds''. The process applied to increase of F-1 hybrids permits production of ''artificial seeds'' (encapsulations) in 1/2 to 1/10 the time it would take to produce natural hybrid seeds. Techniques involving freeze storage and cell suspensions are disclosed. All representative cultivars were regenerated. |
