发明名称 |
Isolation of unreduced oligosaccharides |
摘要 |
A preparative scale method for the isolation of unreduced oligosaccharides of glycoproteins and glycohormone having a N-linked oligosaccharide structure is disclosed. The method employs hydrazinolysis of the glycoprotein or glycohormone under reaction conditions to cause cleavage at the N-linked sites, producing a mixture having as a major component a de-N-acetylated hydrazone derivative of the oligosaccharides, followed by N-acylation of the hydrazone derivative, acid-catalysis of the hydrazone derivative to produce unreduced oligosaccharides, and subjecting the resulting unreduced oligosaccharides to cellulose column chromatography to remove contaminants and to recover the unreduced oligosaccharides.
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申请公布号 |
US4736022(A) |
申请公布日期 |
1988.04.05 |
申请号 |
US19860929962 |
申请日期 |
1986.11.12 |
申请人 |
MONSANTO COMPANY |
发明人 |
RADEMACHER, THOMAS W.;DWEK, RAYMOND A. |
分类号 |
A61K35/14;A61K35/37;A61K35/54;A61K38/16;A61K38/55;C07H1/08;C07H3/06;C07H5/00;C07H5/06;C07H13/06;C07K9/00;C13K13/00;(IPC1-7):C07H19/00;C07H21/00 |
主分类号 |
A61K35/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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