| 摘要 |
<p>A process for detecting ligand-ligand interactions such as antigen-antibody, hapten-antibody, hormone-receptor, protein-nucleic acid, and nucleic acid-nucleic acid utilizing a coelenterate-derived luciferase or a coelenterate--derived photoprotein which is a bioluminescent, low molecular weight, single polypeptide chain as a label in a bioluminescence-linked immunoassay is disclosed. The disclosed process is rapid and process provides greater sensitivity over existing processes. Also, the disclosed process utilizes labels and reagents which possess a shelf life of years. Three types of bioluminescence-lined immunoassays are disclosed: heterogeneous assay requiring a single separation step, homogeneous assay without separation step requiring dual wavelength measurements, and a homogeneous assay without a separation step requiring a single wavelength measurement.</p> |
