发明名称 |
GLYCOPROTEIN gp105 ON BL3 HEMATOPOIETIC STEM CELLS |
摘要 |
An N-glycosylated glycoprotein, having the molecular weight of 105 kDa when glycosylated and 85 kDa when non-glycosylated, is present on the surface of BL3 hematopoietic stem cells, but is absent from the surfaces of other cells including 32D and FDC-P1 myeloid progenitor cells, EL4 T-cells and 3T3 fibroblasts. Antibody which binds the gp105 protein inhibits the proliferation of BL3 cells that occurs in BL3-conditioned medium (BL3CM). It also can inhibit preferentially the formation of fetal liver cell-derived, multi-lineage colonies in vitro, and the development of bone marrow cell-derived, spleen cells colony-forming unit foci in vivo. Anti-gp105 antibody can be employed in making a preparation that is enriched for hematopoietic stem cells. Substantially purified from other proteins, the gp105 protein itself is a useful reagent for isolating and characterizing the factor(s) responsible for the proliferative effect of BL3CM.
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申请公布号 |
WO9639628(A1) |
申请公布日期 |
1996.12.12 |
申请号 |
WO1996US08623 |
申请日期 |
1996.06.06 |
申请人 |
STEMCELL THERAPEUTICS L.L.C. |
发明人 |
WONG, PETER, M., C.;CHUNG, SUI-WAH;HAN, XIAODONG |
分类号 |
C07K14/705;C12N5/0789;G01N33/569;(IPC1-7):G01N33/53;G01N33/543;G01N33/545;G01N35/08;C12P21/04;C12N5/00;C07H19/00;C07H21/00;C07K1/00;C07K14/00;C07K16/00;C07K17/00 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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