| 摘要 |
PURPOSE:To efficiently and industrially enable production of glycoside of phenols, by carrying out a plant tissue culture in the coexistence of phenols by adjusting concentrations of cultured cell and phenols to specific concentrations. CONSTITUTION:When a plant tissue culture in the coexistence of phenols is carried out, concentrations of cultured cell and phenols in a liquid medium are adjusted to be respectively >=250g/l (based on fresh wt.) and 5-20mM/l to produce glycoside of phenols. In order to continuously preferably carry out above-mentioned production, the cultured above-mentioned production, the cultured cell is continuously propagated and incubate, beforehand in a cell growth zone under updated conditions of culture medium, so that a cultured cell concentration in a liquid medium become 100-500g/l (based on fresh weight). Then, the cultured cell is continuously subjected to tissue culture, adjusting the resultant cultured cell concentration and concentration of phenols to be, respectively, >=250g/l (based on fresh wt.) and 5-20mM/l. |
