PURIFICATION PROCESS OF TPA

摘要

Tissue plasminogen activator (tPA) species having a molecular weight of about 70,000 daltons is isolated in a purified form from a crude tPA preparation containing various tPA species having different molecular weights, by bringing the crude tPA preparation into contact with a cation exchanger. The desired tPA species is selectively separated from the cation exchanger by means of a salt gradient elution method.