Preparation of enzymes having altered activity.

摘要

The invention relates to altering the transesterification rate/hydrolysis rate ratio and nucleophile specificity in reactions that are catalyzed by enzymes with catalytic triads such as serine hydrolases and cystine hydrolases by using a novel method to modify the enzyme. Modifications to the enzyme are achieved by altering amino acid residues within 15 angstroms of the catalytic triad in the enzyme.