发明名称 |
Cloning and expression of acidic fibroblast growth factor. |
摘要 |
<p>Unique genes coding for the amino acid sequence of bovine acidic fibroblast growth factor (aFGF) and human aFGF are constructed. The bovine gene is derived from reverse translation of the aFGF amino acid sequence while the human gene is derived by specific point mutations of the bovine gene. Each gene construct is inserted into an expression vector which is used to transform an appropriate host. The transformed host cells produce recombinant aFGF (r-aFGF), human or bovine, which is purified and has an activity equivalent to the native protein.</p> |
申请公布号 |
EP0259953(A1) |
申请公布日期 |
1988.03.16 |
申请号 |
EP19870306066 |
申请日期 |
1987.07.09 |
申请人 |
MERCK & CO. INC. |
发明人 |
LINEMEYER, DAVID L.;KELLY, LINDA J.;THOMAS, KENNETH A., JR.;GIMENEZ-GALLEGO, GUILLERMO |
分类号 |
C12N15/09;A61K38/00;A61K38/22;A61P17/00;A61P43/00;C07K1/16;C07K1/20;C07K1/22;C07K14/00;C07K14/50;C12N1/20;C12N1/21;C12P21/02;C12R1/19;(IPC1-7):C12N15/00;C07K13/00;C07H21/04;A61K37/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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