| 摘要 |
A method for assaying complement fragment C3a, C4a or C5a or the des-Arg derivative thereof in a biological sample which comprises combining equal volumes of the biological sample and a solution of 0.8 to 1.6% of an acridine derivative selected from the group consisting of acrinol, acriflavine, acriflavine hydrochloride, and aminacrine, incubating the mixture for about one minute to 2 hour at about 25 DEG C., recovering the supernatant from the resultant precipitate, incubating the supernatant with a known amount of a labeled complement fragment selected from C3a, C4a or C5a or the des-Arg derivative thereof and a known amount of antibody which recognizes said complement fragment or des-Arg derivative thereof, separating the free labeled complement fragment from the bound labeled complement fragment, measuring the amount of labeled complement fragment in either the free or antibody bound complement component, and determining the concentration of complement fragment or the des-Arg derivative thereof in the biological sample by comparison to a standard curve and a mercantil kit useful in performing said method.
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