DYE MARKER DUE TO STREPTOMYCETES PLASMID

摘要

Subjecting Streptomyces coelicolor DSM 3030 to total digestion of the complete DNA, cloning into a suitable vector and transformation of a Streptomycetes recipient strain result in colonies with an intense blue-black colour. Reisolation of the plasmid DNA and cutting with BamHI results in a 5.5 kb fragment on which the pigment gene is located. The latter is suitable as marker, in particular as inactivation marker, in Streptomycetes.