| 摘要 |
<p>PURPOSE:To produce an anti-amyloid A protein monoclonal antibody, by subjecting splenocyte to immunized animal and mammalian myeloma cell to cell fusion to give a hybridoma cell strain having peptide activity and cultivating the strain. CONSTITUTION:A mammal is immunized against a peptide shown by the formula His-Ala-Arg-Gly-Asn-Tyr-Asp-Ala-Ala-Lys-Arg or against a bonded material of carrier protein thereof, Splenocyte of the immunized animal and a mammalian myeloma cell are subjected to cell fusion to give a hybridoma cell strain, from which a hybridoma cell strain to be reacted with the peptide is selected and the cell strain is cultivated in a medium or administrated to mammalian abdominal cavity and made into ascites. A monoclonal antibody is accumulated in the culture mixture or in the ascites and the monoclonal antibody is collected.</p> |
