| 摘要 |
<p>A recombinant DNA technique for evaluating the strength and efficiency of promoters in a transient assay for gene expression. A method of evaluating a promoter capable of functioning in a given host cell which comprises: providing, on a plasmid vector substantially incapable of replicating in said host cell, a first genetic sequence to be evaluated for promoter activity operably linked to a second genetic sequence which codes for a reporter molecule secretable from said host cell without the lysis of said host cell, measuring the amount of reportable molecule expressed by said vector and secreted from said cell, and evaluating of said promoter activity as a function of said amount.</p> |
