Expression of g-csf and muteins thereof and their use.

摘要

Human recombinant G-CSF (hr-G-CSF), an mRNA fraction encoding hr-G-CSF, a DNA sequence encoding hr-G-CSF, modified DNA sequences encoding hr-G-CSF, that are expressed at high levels in recombinant hosts are disclosed. Modifications to the 5 min DNA sequence encoding mature mG-CSF are also disclosed. The modifications to the third position nucleotide of at least one in the 5 min region codon of the DNA sequence of G-CSF result in enhanced expression of mG-CSF protein in recombinant host cells, without changing the amino acid sequence of the protein. Other modifications yield muteins of G-CSF in which the NH2-terminal amino acid is not methionine when produced from a recombinant host cell. Expression vectors carrying the altered mG-CSF DNA sequence are disclosed and claimed.