Long wavelength thiol-reactive fluorophores

摘要

Reactive fluorescent dyes compositions and methods of using same are disclosed. Coumarin nucleus or aza coumarin nucleus dyes are disclosed having thiol-reactive groups. Coumarin nucleus or aza coumarin nucleus dyes are disclosed that exhibit a fluorescence emission of at least about 575 nm. Biosensors are disclosed having a binding protein and a Coumarin nucleus or aza coumarin nucleus.