Methods and devices for detection and measurement of analytes

摘要

Sensors for target entities having functionalized thereon, at least one aptamer specific to the target entity, and methods of making and using the same are described for use in glycated protein monitoring and/or biomarkers.

主权项

1. A method for forming a sensor for one or more analytes, comprising:
a) adsorbing binary components comprised of 3-mercaptopropionic acid (MPA) and (1-mercapto-11-undecyl)tri(ethylene glycol) (PEG3) on a substrate; b) reductively desorbing MPA from the substrate of step a); c) forming a dithiobis-N-succinimidyl propionate (DTSP) layer on the substrate of step b); d) immobilizing at least one type of aptamer on the substrate of step c); and, e) removing unbound aptamer from the PEG3 on the substrate of step d), thus leaving aptamer attached to the DTSP layer of the substrate.