Depletion of plasma proteins

摘要

This invention relates to methods of analysis, and in particular to methods for the preliminary fractionation of samples in which low abundance molecules of interest, for example proteins, polysaccharides or fatty acids, are present together with more abundant molecules of little or no interest. In particular, the invention relates to methods of depletion of high abundance proteins from biological samples. Products and kits for use in the method are also disclosed, and form part of the invention. In one aspect, the invention provides a method of depleting a high-abundance molecule from a biological sample, comprising the steps of a) subjecting the sample to affinity depletion using an affinity support with high affinity for a high abundance molecule, and/or b) immunodepletion using an affinity support coupled to an antibody directed against whole or previously fractionated plasma or serum.

主权项

1. A method of depleting a high abundance molecule from a biological sample, comprising the steps of:
a) subjecting the sample to affinity depletion using an affinity support with high affinity for the high abundance molecule; and b) immunodepleting the sample using an affinity support coupled to a chicken IgY antibody, wherein the chicken IgY antibody is a second generation or higher antibody raised against whole blood, serum or plasma or against whole blood, serum or plasma that has already been subjected to at least one round of affinity depletion or immunodepletion, and wherein the chicken IgY antibody binds a plurality of different high abundance molecules.