Fluorescence observation device

摘要

Provided is a fluorescence observation device including: a processor configured to: extract, as a candidate region of interest, a region in a corrected fluorescence image that has a gradation value larger than a gradation-value threshold, the gradation-value threshold being set based on an average of gradiation values, a standard deviation, a first coefficient, and a second coefficient; receive a result, inputted by an observer, as to whether a candidate region of interest displayed on a display is a result of right indicating a determination by the observer that the candidate region of interest is a region of interest or a result of wrong indicating a determination by the observer that the candidate region of interest is not a region of interest; and set at least one of the first coefficient and the second coefficient so as to reflect the result inputted by the observer.

主权项

1. A fluorescence observation device comprising:
a light source configured to radiate excitation light and reference light onto an object; a fluorescence capturing sensor configured to capture fluorescence produced in the object irradiated with the excitation light; a return light capturing sensor configured to capture return light from the object irradiated with the reference light; and a processor comprising hardware, wherein the processor is configured to:
generate a fluorescence image based on the fluorescence captured by the fluorescence capturing sensor;generate a reference image based on the return light captured by the return light capturing sensor;generate a corrected fluorescence image by correcting the fluorescence image with the reference image;set a gradation-value threshold based on an average of gradation values of the entire corrected fluorescence image, a standard deviation of the gradation values, a first coefficient related to weighting of the average of the gradation values of the entire corrected fluorescence image, and a second coefficient related to weighting of the standard deviation of the gradation values;determine and extract, as a candidate region of interest, a region in the corrected fluorescence image that has a gradation value larger than the gradation-value threshold;control a display to display the candidate region of interest extracted and the reference image in association with each other; andreceive a result, inputted by an observer, as to whether the candidate region of interest displayed on the display is right or wrong, wherein a result of right indicates a determination by the observer that the candidate region of interest is a region of interest, and a result of wrong indicates a determination by the observer that the candidate region of interest is not a region of interest,wherein the processor is configured to set at least one of the first coefficient and the second coefficient so as to reflect the result inputted by the observer.