发明名称 |
GLUCOSE ISOMERASE GENE, RECOMBINANT DNA CONTAINING THE GLUCOSE ISOMERASE GENE AND MICROORGANISM TRANSFORMED BY SAID RECOMBINANT DNA |
摘要 |
PURPOSE:To enable mass-production of glucose isomerase, by separating a gene coding a glucose isomerase originated from actinomycetes and clarifying the structure of the gene. CONSTITUTION:A polymeric DNA of an actinomycete cell is completely digested with a restriction enzyme Bam H1 and a DNA fragment fraction having a chain length of about 5.7kb is separated by neutral sucrose density-gradient centrifugal separation process. The fragment is linked to Bam H1 site of a pUC vector using a ligase and transformed to E.coli to obtain a DNA library. The glucose isomerase gene is selected from the library by using a probe consisting of an oligonucleotide labeled with <32>P. |
申请公布号 |
SE8800331(D0) |
申请公布日期 |
1988.02.02 |
申请号 |
SE19880000331 |
申请日期 |
1988.02.02 |
申请人 |
DIRECTOR OF NATIONAL FOOD RESEARCH INSTITUTE |
发明人 |
C * FUKAZAWA;K * KAINUMA |
分类号 |
C12N1/16;C12N1/20;C12N1/21;C12N9/92;C12N15/09;C12R1/125;C12R1/19;C12R1/545;C12R1/865;(IPC1-7):C12N15/00 |
主分类号 |
C12N1/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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