Method of preparing integral multilayer analytical element.

摘要

A method of preparing an integral multilayer analytical element such as for analysis of calcium comprising a water-impermeable light-transmissive support, a reagent layer containing a water-soluble indicator capable of reacting with an analyte to produce an optically detectable change, and a porous spreading layer containing a spreading action controller and/or an acid capable of decomposing the calcium compounds in a sample, superposed in this order, which is characterized in that dissolving the spreading action controller and/or the acid in an organic solvent which does not dissolve the above water-soluble indicator, incorporating this solution into the porous spreading layer, and drying this superposed material.