| 摘要 |
<p>PURPOSE:To achieve high growth performance in cell culture, by culturing a cell in a culture device consisting of a porous membrane having homogeneous pectinated network structure having pectinated rectangular shape encircled with micro-fibril and a knot part composed of stacked lamellas. CONSTITUTION:A porous membrane made of a hollow fiber having a wall thickness of 1-1,000mum and inner diameter of 0.01-10mm is produced from a polymer such as crystalline polyethylene, polypropylene, etc., by draw pore- forming process. Preferably, the core diameter of the membrane is 0.1-2.0mum and the porosity of the membrane is 50-80%. When the membrane is observed by a scanning electron microscope, the areal ratio of the knot part in the field is preferably 10-30%. The average length and diameter of the micro-fibril are controlled to 0.1-6.0mum and 0.02-0.3mum, respectively, A cell is placed outside of a hollow fiber partitioned with said porous membrane and a culture liquid is circulated through the hollow part from one opening to another opening to enable the supply of nutrients and oxygen or the removal of product or waste produced by the cultured cell.</p> |
