A process for producing bovine lactoferrin in high purity.

摘要

A process for producing bovine lactoferrin in high purity from raw milk-materials containing skim milk or whey originated from cow's milk which comprises: adsoption step wherein said raw materials are contacted, at a temperature between 0 - 60 DEG C, with weakly acidic cation-exchanger which includes carboxymethyl groups as ion exchanging groups and has haemoglobin adsorption property more than 3.5 g/ 100 m l ; rinsing step wherein said exchanger is washed with water to remove substances other than those adsorbed to said exchanger; and desorption step wherein the substances adsorbed to said exchanger are desorbed therefrom with a solution of one or more of salts to thereby yield highly purified bovine lactoferrin without deterirating raw milk materials. Said desorption step can be carried out in a single stage to produce lactoferrin more than 80 % purity to total proteins recovered, and in two stages to attain more than 98 % purity.