| 摘要 |
<p>Thrombin-binding substances are obtained by fractionating human urine by ion-exchange chromatography, affinity chromatagraphy using a thrombin-bound carrier, immune adsorption column chromatography, gel filtration, and/or molecular-weight fractionation. One of the substances has a molecular weight of 46,500 +/- 6,000 in reduced condition and 39,000 +/- 10,000 in unreduced condition by SDS PAGE and an isoelectric point at pH 5,0-5,3, while the other has a molecular weight of 40,000 +/- 8,000 in reduced condition and 31,000 +/- 10,000 in unreduced condition by SDS PAGE and an isoelectric point at pH 4,9-5,7. They have strong affinity to thrombin. They are capable of promoting the thrombin catalyzed activation of protein C and prolong clotting time. They are stable to denaturing agents (urea and sodium dodecylsulfate).</p> |
