| 摘要 |
<p>For the genetic production of proteins a vector is introduced into Gram-negative host cells, said vector containing at least one gene coding for the desired protein; the gene is transcribed and translated. For the extracellular production of the protein its coding gene is inserted into a vector which contains the IgA-protease precursor gene from micro-organisms of the family Neisseria in such a way that the coding gene is positioned inside the sequence of the IgA-protease precursor gene. A plasmid which is particularly suited for this is plasmid pIP100 contained in E. coli DSM 3775.</p> |
