| 摘要 |
<p>Expression and secretion vector in yeasts, useful for preparing heterologous proteins, comprising a synthetic oligonucleotide, which directs the secretion of the heterologous protein, positioned between the inducible hybrid promoter GAL-CYC and a multiple-site polyinker followed by the signals of transcription termination recognized by the RNA-polymerase of the yeasts. Hybrid plasmid obtained by cloning in one of the restriction sites of the polyinker of said vector, the DNA sequence which codes for a heterologous protein. Process for the preparation of heterologous proteins, which comprises cultivating, in a suitable culture medium, a yeast transformed with said hybrid plasmid, and recovering from the culture medium the so-obtained proteins. The so-obtained proteins comprise hormones, lymphoquins, viral antigens or immunogens, useful in the therapeutical and diagnostic field.</p> |
