| 摘要 |
PURPOSE:To obtain the titled agent having high suppressing effect in high purity, by culturing and proliferating a malignant tumor cell of an animal, culturing the cell in an extraction medium, removing the malignant tumor cell from the cultured liquid, eluting the liquid with a specific column and collecting a fraction eluted at a specific time. CONSTITUTION:A malignant tumor cell of an animal including human (e.g. established cell strain H.R.C originated from human nephrocyte cancer cell) is cultured and proliferated, transplanted to a medium for extraction (e.g. serumfree medium) and cultured at 35-37 deg.C for 4-7 days. The proliferated malignant tumor cell is removed from the medium to obtain a cultured liquid for extraction. The crude product is dissolved in methanol of an amount of 1/25 of the stock liquid and the supernatant liquid is eluted with a column filled with a fixed phase of 10cm in diameter and 120cm long and composed of hydroxypropylated dextran gel bead at a flow rate of 800ml/hr. The fraction eluted in 5.625-7.25hr is collected to obtain the objective agent for suppressing the proliferation of malignant tumor cell. |
