BIOSENSOR

摘要

PURPOSE:To permit the extremely easy measurement of a substrate concn. in a vital sample by integrating an insulating substrate, electrode system and a porous body carrying oxygen reduction enzyme and electron receptor. CONSTITUTION:A conductive carbon paste is printed by screen printing on the insulating substrate 1 and is dried by heating to form the electrode system consisting of a counter electrode 2, a measuring electrode 3 and a reference electrode 4. The insulating paste is then printed thereon to partially cover the electrode system and to leave 2'-4' which are the electrochemically working parts of the respective electrodes and is subjected to a heat treatment to form an insulating layer 5. The parts 2'-4' are then subjected to a heat treatment in air after grinding. A holding frame 6 bored with a hole is adhered to the insulating layer 5 and the porous body 7 is so held in the hole as to cover the electrode systems 2'-4'. A cover 8 having the aperture part smaller in diameter than the body 7 is further adhered thereto to integrate the entire part. The specific components in the vital sample are easily quantitatively determined with high accuracy in the above-mentioned manner.