| 摘要 |
PURPOSE:A plasmid recombinant producing protein showing epidermal growth factor (EGF), efficiently, having a structural gene to code a specific polypeptide inserted into the plasmid recombinant. CONSTITUTION:A DNA base sequence of a structual gene of protein factor (EGF) having epidermal growth activity and a translation control signal range is synthesized to give ECF gene (A) having a recognition breakage site of restric tion enzyme XbaI between a gene codon at the top of the structural gene and SD sequence. Then manifestation plasmid vector pDR540 having tac promoter is scissored with BamHI to give cleft cloning vector (B). The components A and B are linked with ligase T4 to give recombinant plasmid pPGFLI (C). Then the component C is transduced into Escherichia coli JM103, the prepared transformant is cultivated and multiplied. Then the multiplied mold is extracted and separated to give a recombinant plasmid (figure) into which a structure gene to code a polypeptide consisting of 53 amino acids having amino acid in the downstream of the tac promoter is inserted. |
