| 摘要 |
PURPOSE:To obtain a saturated carbonyl compound useful as chemical, food additive, etc., in high yield, in high selectivity and inexpensively, by treating an alpha,beta-unsaturated carbonyl compound with a specific bacterium to reduce the compound with hydrogen. CONSTITUTION:Clostridium thermosaccharolyticum A-46 strain is cultivated in a medium containing saccharide, etc., at pH 5-8 at 45-65 deg.C for 4-36hr to give a culture mixture, which is treated to give a mold. Then an alpha,beta-unsaturated carbonyl compound shown by formula I [R<1> is H, lower alkyl, -CN-, -NHCHO or halogen; R<2> is H, (cyclo)alkyl, alkenyl, etc.; R<3> is H, lower alkyl or alkoxy], formula II [R<4> is R<3>; Y is (CR<5>R<6>); n is 3-16; R<5> and R<6> are H, lower alkoxy, halogen, etc.] or formula III (R<7> and R<8> are H or alkyl; m is 2-4) is brought into contact with the mold and pure hydrogen, etc., and hydrogenated at 45-65 deg.C at pH 6-8 at 0.1-20atm. for 0.5-2hr to give a saturated carbonyl compound. |
