PROTECTED BINDING ASSAY

摘要

A specific binding enzyme-resistant ligand assay test material is provided, which material comprises (a) a solid phase incorporated with one partner of a specific binding pair comprising said ligand or a binding analog thereof and a specific binding protein therefor; (b) a conjugate comprising the other partner of said specific binding pair incorporated with a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme. The invention further provides a specific binding method of assaying for an enzymeresistant ligand in a sample, which method uses the above test material.