发明名称 CLONING VECTORS FOR EXPRESSION OF EXOGENOUS PROTEIN
摘要 <p>The construction of a recombinant DNA cloning vector useful for expressing exogenous protein is described, which comprises (a) a DNA segment containing a functional origin of replication; (b) one or more DNA segments, each of which conveys to a transformable host cell a property useful for selection when said vector is transformed into said host cell; and (c) a DNA segment comprising a sequence that defines, in tandem, (1) the promoter of a lipoprotein expression control sequence, (2) the 5' untranslated region of a lipoprotein expression control sequence and (3) a translation start codon followed, without interposition of a portion or all of nucleotide sequence coding for endogenous protein, by a nucleotide sequence coding for an exogenous protein or by a nucleotide sequence coding for an enterokinase cleavage site to which is joined, without interruption, a nucleotide sequence coding for an exogenous protein.</p>
申请公布号 CA1231068(A) 申请公布日期 1988.01.05
申请号 CA19830428700 申请日期 1983.05.24
申请人 LILLY (ELI) AND COMPANY 发明人 MAYNE, NANCY G.;BURNETT, JAMES P., JR.;BELEGAJE, RAMAMOORTHY;HSIUNG, HANSEN M.
分类号 A61K38/27;C07H21/04;C07K14/575;C07K14/61;C12N15/00;C12N15/09;C12N15/62;C12N15/70;C12P19/34;C12P21/02;C12R1/19;(IPC1-7):C12N15/00 主分类号 A61K38/27
代理机构 代理人
主权项
地址