Detecting polynucleotide sequences

摘要

A method of detecting a target polynucleotide sequence in a sample uses a labelled polynucleotide secondary probe, and a polynucleotide primary probe having sequences complementary to both the target and the secondary probe. The sample, immobilized or in solution is hybridized with the primary probe; and the labelled secondary probe is also hybridized with the primary probe. The method permits the production of a labelled secondary probe which can be used in conjuction with many different primary probes for different hybridization reactions.