Hybridomas producing monoclonal antibodies specific for polypeptide fragments derived from human Factor VIIIC are provided. Class I hybridomas produce monoclonal antibodies reactive with a 80/77 kd doublet fragment or with both the 80/77 kd doublet and a 240 kd polypeptide. Class III hybridomas produce monoclonal antibodies reactive with the 240 kd polypeptide as well as a 92.5 kd fragment and its precursors. Class III antibodies show additional reactivity with a 40 kd thrombin digestion product. The monoclonal antibodies are useful for the separation of Factor VIIIC and its constituent polypeptides, as well as for the immunoassay of Factor VIIIC in biological samples.
申请公布号
US4716117(A)
申请公布日期
1987.12.29
申请号
US19850689274
申请日期
1985.01.07
申请人
CHIRON CORPORATION;NORDISK GENTOFTE
发明人
KUO, GEORGE;MASIARZ, FRANK R.;TRUETT, MARTHA;VALENZUELA, PABLO;RASMUSSEN, MIRELLA E.;FAVALORO, JENNIFER