| 摘要 |
PURPOSE:To measure a plurality of antigens in the same sample at a high sensitivity simultaneously, by using a labelled antibody produced by bonding an antibody to a phycobiliprotein as fluorochrome protein. CONSTITUTION:The bonding of an antibody to a phycobiliprotein is executed by an ordinary method. A bonding is performed between R-phycoerythrin and an anti-human albumin antibody, C-phycocyanine and an anti-human immunoglobulin G antibody, allophycocyanine and an anti-humam immunoglobulin A antibody. On the other hand, three kind of antibodies not labelled are immobilized on respective carboxylated polyacryl amide gel beads. These three kind of bead suspensions are taken into equivalent test tubes and a mixed reference liquid of an antibody to be inspected is added to each of the test tubes and the supernatant is thrown away after a reaction. Liquids of antibodies labelled of said three kinds are added to the beads to react, after a washing and separation are conducted, the beads are suspended in a buffer. The intensity of fluorescence is measured using excitation wavelength and emission wavelength suitable for individual phycobiliproteins.
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