BACILLUS GENUS CELL HAVING RECOMBINANT DNA AND PRODUCTION OF ALKALI CELLULASE USING SAME

摘要

PURPOSE:To impart high alkali cellulase productivity to a bacterial cell, by integrating a DNA fragment carrying a genetic information of alkali cellulase and introducing the resultant recombinant plasmid into a bacterial cell of Bacillus genus. CONSTITUTION:An alkali cellulase gene fragment is separated from Bacillus sp-N-2 (FERM P-8809) which is an alkalophilic bacteria producing alkali cellulase. A DNA fragment carrying the genetic information of alkali cellulase is integrated in a plasmid vector. The recombinant plasmid is introduced into e.g. Bacillus subtilis RM125 strain to obtain a microbial strain. The strain is cultured and the alkali cellulase accumulated in the cultured product is separated therefrom.