| 摘要 |
An immunoassay for determining qualitatively or quantitatively the presence of an analyte in a sample by means of specific binding, wherein the sample is contacted with a moveable solid phase carrier material, e.g. magnetic particles (620) on which is immobilised a first binding reagent having specificity for the analyte and with a labelled reagent which can participate in either a "sandwich" or a "competition" reaction with the first reagent in the presence of the analyte and following an incubation period sufficient to allow the reaction to take place, the carrier material is moved within the assay medium (618) to a location adjacent a signal sensing means (602), the label generating a signal, such as chemiluminescent light, continuously in the assay medium and the magnitude of the signal generated in the vicinity of the sensing means being used as a measure of the extent to which the binding reaction has occurred. Preferably the assay medium incorporates a masking or quenching agent which suppresses signal generated in regions of the assay medium remote from the signal sensing means. |
