| 摘要 |
PURPOSE:To efficiently obtain D-pantolactone, by reacting DL-pantolactone with a special hydrolase to hydrolyze only the L-isomer. CONSTITUTION:A hydrolase of a microorganism belonging to the genus Sporidiobolus, preferably Sporidiobolus Johnsonii IFO6903 or a microorganism belonging to the genus Sterigmatomyces, preferably Sterigmatomyces elviae IFO1843 is reacted with DL-pantolactone. The reaction is preferably carried out under condition of 10-300g/l DL-pantolactone concentration, 10-50 deg.C temperature and 3-7.5pH. A phosphoric acid buffer solution, alkali, e.g. NaOH, etc., CaCO3, etc., are preferably added for maintaining the pH. After completing the reaction, the D-pantolactone can be separated and collected by operation, e.g. fractional crystallization, solvent extraction etc. |
