| 摘要 |
PURPOSE:To enable the detection of antibody excess and antigen excess and to easily obtain correct values by changing the antigen quantity or antibody quantity in a reagent and making a 2nd measurement after a 1st measurement and comparing the results of the two measurements. CONSTITUTION:Two kinds of concns. C1, C2 in the case of the antibody excess and antigen excess are obtd. with the same measured value Va if the 1st measurement is first made in order to determine the antigen concn. in the sample by the measurement of absorbancy. The antigen quantity or antibody quantity in the sample or reagent is then changed and the 2nd measurement is made, by which the measured absorbancy value Va' is obtd. For example, the result of the comparison made between Va and Va' when a specified quantity of the antigen is added to the sample reveals that the absorbancy increases with an increase in the antigen concn. in the antibody excess region in the case of 1) Va<Va'. The results, therefore, indicates that the sample is in the antibody excess region and that the concn. C1 is consequently the correct concn. value. The absorbancy decreases with an increase in the antigen concn. in the case of 2) Va>Va', which indicates that the sample is in the antigen excess region and that the concn. C2 is consequently the correct concn. value.
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