| 摘要 |
<p>A process for labelling DNA fragments (oligonucleotides) using fluorescent dyes. The labelling is carried out by converting the DNA fragment into its 5'-[HS-(Y)z]-derivative, and the latter is reacted with a derivative of a fluorescent dye, which together with the 5'-[HS-(Y)z]-derivative forms a DNA fragment of the formula (I) fluorophore-X-S-(Y)z-CH$(2,6)$ -oligonucleotide, wherein X and Y independently are a group comprising one or more carbon atoms and/or hetero atoms, z = 0 or 1, and fluorophore is the residue of a fluorescent dye. In a preferred embodiment the DNA fragment is converted into its 5'-(S-triphenylmethyl-3-mercaptopropyl phospho)-derivative and the 5'-(3-mercaptopropyl phospho)-derivative obtained after cleavage of the triphenylmethyl group is reacted with a (5- and/or 6)-iodoacetamino-rhodamine or -fluorescein.</p> |
