| 摘要 |
<p>A biologic response modifier (BRM) substantially free of endotoxin, intact cells, cell walls, and cell membrane fragments, which comprises natural membrane vesicles and ribosomes in a suspending buffer. Membrane vesicles are comprised of cellular membrane material endogenous to a selected microorganism which does not evoke a significant immune-deviating response in patients and is substantially non-pathogenic in humans. The ribosomes are also endogenous to the selected microorganism, which is one in which membrane vesicles can be formed from cell membrane material. The BRM, readily endocytosed by the patient's monocyte-macrophage cell line, is produced by cultivating cells of strain Serratia marcescens, harvesting cultivated cells, preferably dissociating endotoxin with appropriate detergent, treating cellular concentrate to produce ribosomes and to produce membrane vesicles with diameters < 110 nm, separating the ribosomes and membrane vesicles, and resuspending them in appropriate buffer such that means particle diameter is > 170 nm.</p> |
